Introduction Prostate cancer development and early onset disease is driven by aberrant androgen aling via the androgen receptor AR activity for growth promotion and apoptosis inhibition.
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The process of EMT is an indispensable developmental program for implantation, embryogenesis and organogenesis, but also cancer initiation and progression Kalluri and Weinberg, We found that such a combination strategy effectively suppressed CRPC via navigating cycles of EMT and changes in the cytoskeleton integrity.
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Louis, MO or R Antibodies The following antibodies were used for the various experiments. Cell viability assay The effect of the various treatments on prostate cancer cell viability was evaluated using the Thiazolyl Blue Tetrazolium bromide MTT assay.
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Luciferase reporter gene assays LNCaP95 cells 1. All transfection assays were performed in at least three independent experiments in triplicate wells.
Prostate tumor xenografts were harvested two days after the last treatment and tissue specimens were subjected to histopathological analysis. After blocking nonspecific binding 1. Louis, MO and counterstained with haematoxylin. ImageJ software was used for calculation of densitometry.